Fluorescent microscopy: ine tsvakurudzo

Shoko rokuti "maikorosikopu" rine midzi Greek. In shanduro zvinoreva kudzidza zvinhu dzakakwirira-zvakarurama zviridzwa. . Mumakore achangopfuura kuwedzera dzakakurumbira fluorescent uye Erekitironi microscopy.

chisarudzo

Pasi payo, sezvo mutemo, kunzwisisa shoma daro panguva iyo ingava zvakajeka distinguishable zvinhu. Musi chisarudzo anokwanisa zvokushandisa mwero kupinda kuchaenderana munyika roga nyika, unogona kuona kukura chinhu ichocho. With guru kuwedzera miganhu zvivako vanogona batanidza. Saizvozvowo, pane zvimwe, kupfuura izvo kusvika zvine maatomu akafanana maturo.

The nzira fluorescence microscopy: mashandiro

Kana simba kutendeuka uko absorbs fuma kupinda zvinooneka nemwaranzi kunoitika Naiwo mufaro. Inonzi luminescence. Fani iyi imhaka yokuti zvimwe zvinhu pasi pokudzora chiedza kutanga emit mwaranzi nevamwe (kazhinji yakakura) wavelength. Uyewo, zvimwe zvinhu kuti vari vakakwana chiedza mamiriro wainyatsotaura ruvara pasi pokudzora ultraviolet chiedza Kuchinja ruvara rwayo.

Specificity

Chinhu kuti haichaonekwi pasi UV chiedza, unogona emit yakajeka kubwinya, kana kubatwa rinokosha fuma. In zvinhu izvozvo mavara akasiyana chiedza murima. vanopenya kuchinyanya yakasiyana, asi somunhu mutemo, iduku. Panyaya iyi, fluorescence microscopy unoshanda mune rima nzvimbo. Kana kushandisa ichi mhando kutsvakurudza unogona kuonekwa nechiedza chinhu, raakanga anoratidza. Makemikari anoumba munyama, masero huchachinja unhu pakudzidza. Fluorescence microscopy anoonekwa kuna pamwero histochemical kudzidza.

kupatsanura

может быть первичной или вторичной. Fluorescence microscopy inogona chikuru kana yechipiri. Mukupera nyaya, chinhu chinogadzirwa ne chaiwo yeimwe kupa luminescence. основывается на собственной способности исследуемого элемента излучать свет. Primary fluorescent microscopy kwakavakirwa ravo nemano ane chinhu kuti emit chiedza.

midziyo

Fluorescence microscopy rinoitwa kushandisa dzakasiyana-siyana namano. The zvivheneko kunoshanda chinoumba yacho chinhu. Zvinonzi vakapakata UV rambi. Mukuwedzera, mudziyo rinoshandisa mamwe filters. Mune dzimwe nhumbi dziri wandei yakasiyana configurations. Zvichienderana iro ruvara inoshandiswa nokuda luminescence excitation - ultraviolet kana zvitema, pakati chiedza tsime uye chinhu kuferefetwa anoiswa zvakakodzera firita. Sezvo luminescence nemaziso maatomu akafanana zvinoshingaira kusimba excitation chiedza, izvozvo achabatwa chete bedzi. Surplus yemwaranzi kuripa kubva kupi kuti ndiparadzemo yero-nyoro firita. It anoiswa pamusoro chiridzwa eyepiece. Chinonyanya raidudzwa mugumisiro luminescence chichaitika kana Sefa unotemwa chose chose mwaranzi yechiedza zvekubuda kubva kupi.

Ndeipi kugadzwa muchiedza anooneka? Mairi pane chiedza kunobva uye zvipenyu maikorosikopu. Between mugirazi chikwata uye rambi akatasva rutema-Violet firita. Izvi zvinogona kuva FS-1, UFS-3 uye zvichingodaro. Yellow Sefa zvinonyatsoenderana pamusoro eyepiece yacho maikorosikopu. Uchibatsirwa yavo, chinhu anowira zvitema-Violet chiedza. Iye fara luminescence. Asi chiedza ichi kunogona kuvhiringidza kuratidza jekeso. Saka, munzira kuenda ziso iye dzakadimburirwa yero firita. Kuisa chiedza chakaitwa Koehler nzira, kunze kwechinhu chimwe chete. Hwidibiro condenser anofanira zvizere yakazaruka. Munguva yokudzidza zvakakosha kushandisa asiri fluorescent mafuta kunyudza. Kuderedza mukati achine inowedzerwa kwachiri nitrobenzene (2-10 anosiya / 1 g).

Fluorescent microscopy: mafomu mu zvidukuduku

Zvakanakira ichi mhando kutsvakurudza vari:

1. Mukana chokuwana ruvara mifananidzo.
2. High musiyano unozvipa richivhenekera zvinhu pamusoro dema background.
3. Kuonekwa uye localization kuti mamwe nemavhairasi uye utachiona.
4. Mukana kudzidza yakajeka uye opaque zvipenyu.
5. Kudzidza zvinotsigira upenyu mune simba ravo.

Zvinokoshawo kuti kutaura kuti fluorescent microscopy kunoita wakanakisisa nzira histology uye cytochemistry, nokukurumidza kuongorora chirwere.