The chikuru nemamiriro puroteni

Protein mamiriro anogona anomiririrwa rimwe mana zvokusarudza. Yega ine peculiarities ayo. Saka, pane quaternary, ternary, yechipiri uye chikuru nemamiriro puroteni.

Wokupedzisira pamwero pazvinhu izvi chinomiririra Linear polypeptide ngetani amino acids. Amino acids vakabatana pamwe peptide zvisungo. The chikuru sechivako puroteni ndiwo nyore nenhanho kurongwa mamorekuru. By covalent peptide zvisungo pakati Arifa-amino boka rimwe amino asidhi uye Arifa-carboxyl boka rimwe Morekuru kunopa kumusoro pakusimba.

In paumbwe peptide zvisungo zviri masero ari kusashanda carboxyl boka rokutanga. Mushure kubatana neboka amino. Inenge chete rabhoritari vaiita polypeptide usage.

Peptide chisungo, inova dzokororo Unit polypeptide cheni ane nhamba zvinhu. Pasi pokudzora izvi zvinhu kwete chete kuumba chikuru nemamiriro puroteni. Ivo chokuita zvose rokumusoro pachezvake yacho polypeptide cheni. Pakati huru chinosiyanisa zvinhu with coplanarity (unyanzvi ose maatomu kuti vanobatanidzwa mune peptide boka iri imwe ndege) panyaya substituents mhiri-N-C linkages, zvivako zviripo 2 resonance siyana. Chaiwo zviri peptide ukama zvinosanganisirawo kukwanisa kuumba rehydrogen zvisungo. Pakazoti neimwe peptide neboka ingangoumbwa vaviri rehydrogen zvisungo pamwe nemamwe mapoka (kusanganisira peptide). Zvisinei, pane kunze. Izvi zvinosanganisira peptide mapoka pamwe mapoka hydroxyproline kana proline amino. Vanogona kuumba chimwe chete rehydrogen chisungo. Izvi zvinokanganisa paumbwe mapuroteni yechipiri mamiriro. Nokudaro, mugove aripi hydroxyproline kana proline, kuti peptide cheni iri nyore akatsunga nokuti kuti pane yechipiri rehydrogen chisungo kuti aizochengeta nayo (sezvo kazhinji).

The zita achiumbwa kubva amino iine acid peptide mazita zvaisanganisira mavari. Dipeptide kupa mbiri amino iine acid tripeptide - matatu, tetrapeptide - mana, uye zvichingodaro. Mumwe polypeptide cheni (kana peptide) chero kureba aripo N-chinouraya amino acid, ine vakasununguka amino boka uye C-chinouraya amino acid, umo yepachena boka carboxyl.

Properties kwemapurotini.

Kudzidza yeimwe izvi, masayendisiti kuziva akawanda nyaya. Vaongorori anonyanya wakatsvaka kuti awane kukura, kuona chimiro uye uremu mapuroteni mamorekuru. Zvinofanira kucherechedzwa kuti zvakanga zvakati omei. The kuoma ndechokuti kutsunga hama samolecular uremu kuwedzera kubika kudziya puroteni mhinduro (sezvo zvinoitwa zvimwe zvinhu) hazvibviri, nokuti kuti puroteni mhinduro kwete kubika. A tsanangudzo chinoratidza zvichienderana achideredza pamusoro oma tembiricha iri mhosho. Uyezve, mapuroteni mu chaiyoiyo fomu nokusingaperi kuwanikwa. Zvisinei, achibatsirwa dzakabudirira nzira, kwakaonekwa kuti samolecular uremu anoda kubva 14 kusvika zviuru 45 uye zvakawanda.

Chimwe maitiro chinokosha pamusoro yeimwe ndiyo fractional salting kunze. Izvi ndizvo voga kwemapurotini kubva mhinduro pashure kuwedzera munyu mhinduro chete kuwanda zvakasiyana.

Chimwe maitiro inokosha ndiko denaturation. Izvi zvinoitika muna kunayisa kwemapurotini pamwe inorema simbi. Denaturation ndiko kurasikirwa dzinongoitika zvivako. Izvi zvinosanganisira kutendeuka mamorekuru zvakasiyana, kuwedzera kutyora polypeptide cheni. Nemamwe mashoko, chikuru mamiriro mapuroteni anoramba kuchinja munguva denaturation.