Chii centrifugation? Definition uye musimboti nzira

Chii centrifugation? Chii nzira inoshandiswa? Shoko rokuti "centrifugation" zvinoreva kuparadzana emura kana particulate zvinhu kuva zvikamu zviduku zvakasiyana-siyana nokuda Centrifugal simba. Yakatakura akabudisa kuparadzana zvinhu zvakadaro kuburikidza nokushandisa dzinokosha mano - centrifuges. Chii nheyo nzira?

centrifugation musimboti

Ngatikurukurei muudzame tsanangudzo. Centrifugation - ndiyo nezvainoita nefuma vakuru-nokukurumidza majana mune unyanzvi chimwe. The chikuru Chikamu chipi centrifuge rotor makabuda izvo unotora cheap car insurance kuti sepombi vane zvinhu zviri pasi nekupatsanurwa kupinda munhu zvikamu zviduku. Panguva vachishandisa kutenderera rotor pa yakawedzera aimhanya ari inoshanda Centrifugal simba. Zvinhu vakaiswa pamuedzo sepombi, akasiyana zvinhu vanoparadzaniswa pamusoro tendero arambe achirema. Somuenzaniso, kubudikidza centrifuging mimwe yacho pasi uye precipitate vaparadzana mvura zviri mukati zvakasimba inoramba.

nzira Author

Kokutanga, yakasvika pakuzivikanwa kuti centrifugation yakadaro, mushure kuedza achiitiswa vanotsvakurudza A. F. Lebedevym. The nzira yakanga yakataurwa vaongorori nechinangwa Pakusarudza Zvinoumba ivhu mvura. Kwekutanga date kushandiswa sedimentation mvura, ichiteverwa kupatsanura kubva zvakasimba ivhu. Development centrifugation nzira kuchaita kutsungirira basa ichi zvikuru nokukurumidza. Nokuda kuparadzana ichi chakava zvichibvira dzokubvisa chakasimba kesimba kubva mushonga zvinhu zviri pakaoma hurumende kuti nyaya kwemaminitsi.

centrifugation nzendo

Differential sedimentation centrifugation kunotanga nezvinhu zviri pasi kuongorora. kugadzira ichi rinowanikwa mune zvinhu-Mugari midziyo. Nokufamba pakugadzirisa particulate nyaya vanoparadzaniswa negiravhiti. Izvi zvinoita kuti agadzirire fuma kuti kuparadzana zvirinani Centrifugal nemauto.

Uyezve zvinhu pamuedzo sepombi vari pasi mupise. In danho iri, shandisa anonzi Perforated ngoma, izvo aida kuti kupatsanura mvura kubva zvakasimba inoramba. Nokufamba zviitiko akapa emadhaka zvinosara pamasvingo centrifuge.

zvakanakira nzira

Ichienzaniswa nedzimwe nzira zvainanga kupatsanurwa wacho zvinhu, zvakadai filtration kana sedimentation, centrifugation runoita kuwana precipitate pamwe shoma mwando chinoratidza. Kushandiswa nzira iyi inobvumira kuparadzana vaparadzane dzakarukwa zvakanaka vakapararira slurry. Zvinoguma kuwana PARTICIPLE kukura 5-10 microns. Chimwe chakanakira chinokosha mukana chamupidigori kuurayiwa ayo achishandisa midziyo yemavhoriyamu diki uye miganhu. The chete drawback nezvenzira anoita kumusoro simba namano.

Centrifugation Biology

In zvipenyu, imwe pfuma kuparadzana kupinda munhu zvinhu Kutenga, kana zvakakodzera, achigadzirira zvinodhaka kuongororwa nemaikorosikopu. Centrifugation rinoitwa pasi kunzwisisa mano - tsitorotorah. Kwakadaro mano kuwedzera cheap car insurance kuti sepombi yaipiwa pamwe mharidzo yokuisira, zvose zvamarudzi ose nemasiraidhi zvakaomesa mamiriro. Kubva centrifuge mudziyo pakutsvakurudza mune zvipenyu kunokanganisa zvakananga kunaka zvinhu vakawana uye maererano chitsama anobatsira mashoko tinoawana kubva kuongorora zvabuda.

Centrifugation achinatsa indasitiri

centrifugation nzira hunokosha kweShanduro mafuta. Pane yeHydrocarbon pfuma zvisina zvakazara dzinounzwa mvura DISTILLATION. Centrifugation zvinoita kubvisa zvakawandisa mvura kubva mafuta, kuwedzera unhu kwayo. Panyaya iyi, mafuta akanga zvazvichanyungudutswa benzene, uye ipapo neshungu ⁶⁰ ° C, uye ipapo pasi munhu Centrifugal simba. Pakupedzisira kuyera huwandu residual mvura acho uye nzira akadzokorora, kana zvichidikanwa.

centrifuging ropa

Nzira iri rinoshandiswa chaizvo somushonga zvinangwa. Mukurapa, hunobvumira kugadzirisa zvinotevera akatevedzana mabasa:

1. The zvichiguma akanatsa ropa ivhu kuti plasmapheresis. Zvinangwa izvi, mumwe centrifuge anosiiwa masero eropa kubva rayo plasma. The oparesheni runoita kuti kubvisa ropa nemavhairasi, kunyanya antibodies, pathogenic utachiona, muchetura.
2. Kugadzirira ropa anyatsoronga kuwedzerwa. Pashure kupatsanura muviri Urume kupinda vakaparadzana zvikamu zviduku kubudikidza centrifugation zvishandiswezve anyatsoronga masero matsvuku eropa uye plasma raishandiswa kuisira kana chando kuti tevera kushandiswa.
3. Voga kuti maplatelet. Fuma anobudiswa kubva yeplasma ropa, platelet-vapfumi. The zvichiguma gobvu inoshandiswa zvomusejari uye hematology madhipatimendi okurapwa masangano, nechimbichimbi kurapwa, oparesheni. Kushandiswa maplatelet mukurapa runoita kuvandudza kweropa iri njodzi.
4. Kwetunhu akazara RBCs. Centrifugation kwamakamuri ropa kunoitika takaparadzana zvayo yaigona zvikamu zviduku maererano nzira inokosha. The rakapedzwa guru yakapfuma erythrocytes, rinoshandiswa vozoriwedzerwa arasikirwe neropa, kushandiswa. Ndokurongedza masero matsvuku eropa inowanzoshandiswa nokuda Mabatirwo kupererwa, zvimwe zvirwere zviri systemic hunhu ropa.

vazhinji chizvarwa chitsva mano zvaishandiswa ano okurapa, izvo zvinoita kuti vapararire ari muhombe Drum kune imwe nokukurumidza uye kuamisa pane imwe nguva. Izvi zvinobvumira kuti zvakarurama kuparadzanisa ropa mukati erythrocytes, maplatelet, plasma, serum uye clots. Saizvozvowo nezvokumwe dzimwe pamuviri fluid kunyanya fuma vanoparadzaniswa mu weti sezvamunoita.

Centrifuges: Marudzi anokosha

Isu afunga chii centrifugation. Zvino ngationei kuti mano zvinoshandiswa kushandisa nzira. Centrifuges dzakavharika uye akazaruka, pamwe zvemaziso kana Chinyorwa hutachiwana. The chikuru kushanda chikamu Chinyorwa rakavhurika mudziyo anoita kutenderera akabatana iri vertically. In aro okumusoro chikamu perpendicularly wakaiswa ngaasungirire, sezvamakaropafadzwa akaronga chinjwa simbi ruoko. Vanoisa rinokosha chubhu, kujekesa nechepasi. Nechepasi dzisimbe twa wadding, iyo haambotauri kuvadza girazi chubhu, neVadzidzi simbi. Uyezve, midziyo inodzoserwa. Kwapera nguva nemvura anosiiwa yakasimba akarembedzwa inoramba. Zvadaro, mukuru centrifuge anomiswa manually. Pazasi zvibereko riri dzichiitika gobvu, yakasimba precipitate. Above ndicho mvura chikamu yacho.

Zvokuimba centrifuge chine gedhi nhaka vakawanda cartridges kutakura sepombi. mano akadaro yamunenge kupfuura Chinyorwa. rotors avo rinosundwa dzemagetsi Motors simba uye anogona mhanyisa kusvika 3,000 RPM. Izvi zvinoita kuti pave Kuparadzana nani emura kubva chikukutu zvinhu.

Zvakakurukurwa kugadzirira bvunzo sepombi kubudikidza centrifugation

Zvibereko, izvo inoshandiswa centrifugation inofanira kuzadzwa bvunzo zvinhu pamusoro fanana uremu. Naizvozvo, nokuti mukuru-zvakarurama kuyerwa dzakananga zviyereso anoshandiswa. Kana uchida kudimburira akawanda sepombi ari centrifuge, akareva inotevera kugamuchira. Kusarudza peya regirazi midziyo uye kuwana mukanyiwa iwoyo, mumwe wavo asara sokunoratidza. Tevera kunambirwa mwero pamwe mharidzo vasati achiisa muchina. chidobi Izvi zvinoita kuti ukurumidze zvikuru basa, kana zvakakodzera, kugadzirira achikanda nhevedzano sepombi.

Zvinofanira kucherechedzwa kuti zvibereko haana kuisa yakawandisa nefuma kuferefetwa. The girazi mudziyo yakazara zvokuti kumucheto daro riri pane 10 mm. Zvikasadaro mashoko richadururwa ari chubhu pasi pokudzora Centrifugal simba.

Sverhtsentrifugi

Nekuti akazvitsaurira zvinoumba zvikuru yakaisvonaka suspensions kukwana kushandisa kwakajairika Chinyorwa kana zvemaziso centrifuges. Muchiitiko ichi kunoda zvinopfuura anoyevedza kukanganisa kunoitwa Centrifugal mauto ari fuma. Kana pakutevedzera pezvakaitwa dzakadaro anechekuita sverhtsentrifugi.

Midziyo hunomiririrwa negomba Drum vakapakata zano iri muchimiro duku dhayamita sepombi - kwete kupfuura 240 mm. Kureba dhiramu zviri kupfuura ayo muchinjikwa chikamu, izvo zvinoita kuti tikwanise kuwedzera zvikuru uwandu muchichinjana uye pave simba Centrifugal simba.

In sverhtsentrifuge bvunzo fuma runoyerera kupinda dhiramu, chinokambaira chubhu uye paricharova anokosha reflectors kuti zvakaraswa zvinhu pamusoro mudziyo. Rinewo kamera, yakagadzirirwa zviparadzanise goho chiedza uye inorema mvura.

Zvazvakanakira sverhtsentrifug zvinosanganisira:

• Mhedziso tightness;
• soro kuchinyanya zvinhu kumwedzi;
• duku;
• tinogona kupatsanura zvinhu panguva samolecular chechetere.

In mhedziso

Saka isu afunga chii centrifugation. Nzira aripo anowana chikumbiro chacho ari kudiwa govera kwemvura zvinogadzirisa yokuchenesa zvinwiwa, kupatsanurwa zvinoumba pakugadzira vanoshingaira kana makemikoro. Nekuti akazvitsaurira zvinhu panguva samolecular chechetere ultracentrifugation anoshanda. centrifugation nzira zvinowanzodavirwa kushandiswa kwemakemikari, peturu, zvenyukireya, chikafu maindasitiri, uyewo mune zvokurapa.