ELISA diagnostics

ELISA diagnostics of infections ndiyo nzira ye enzyme immunoassay , inowedzera kushandiswa mukurapa. Inobva pakuonekwa mumuviri wemurwere wezviratidzo zvinorwisa zvirwere kana kuti kuonekwa kweantigens e-causative agent wechirwere.

Antigens zvinhu izvo, kupinda mumuviri, zvinokonzera kudzivirirwa kwechirwere chemuviri, kana chakatapukirwa, chinotanga kubudisa mapuroteni chaiwo - masimba.

Mumwe nomumwe pathogen ane zvigadziro zvake zveantigens, achizvifunga, chiremba anogona kuita "mufananidzo" wevanhu vanofungidzirwa nezvechibage. Zvirwere zvemuviri zvinoshandawo zvakasarudzwa uye zvinogadzira zvirwere zvehutachiona, uye hazvizobatanirwi nemaantigens eimwe pathogen.

Iyo inogadziriswa neE ELISA diagnostics inogona kuitika maitiro antigens kumagadzirirwo akaunzwa, zvichiguma nekuda kweshoko iri rinopesana noutachiona-antigen rakaumbwa. Ndeipi kupi zvinorwisa zvirwere kana maantijeni zvinobva kune zvakadaro? Zviri nyore kwazvo, zvinogadzirwa zvekushanda kune rumwe rudzi rwekutsvakurudza. Mhando imwe neimwe yeantigens kana antibodies inonyorwa pamiti yacho ine enzyme chaiye. Nokudaro, nekuumbwa kwe-antibody-antigen yakaoma, ichava nehuturo mukugadzirwa kwayo, uye apo reagent yemuvara inowedzerwa, nekuda kwekuvapo kwechiraira ichi, zvose zvinetso zvakasimuka zvinoshandura ruvara.

Apo ELISA inoshandiswa kuongororodza senzera, kuwedzera kwehutachiona hwezvirwere uye maantijeni mune zvishandiswa pakushandurwa kunoongororwa, uye mimwe midziyo inokosha inoverenga nhamba yavo.

Migumisiro ye ELISA

Kana maantijeni ehutachiona awanikwa kana kuti zvirwere zvemasimba kwavari, izvi zvinorevei? Izvi zvinoreva kuti murwere zvechokwadi ane chirwere. Uye IFA kuongorora kunogona kupa zita re microorganism rinokonzera kutaridzika kwei antigen iyi yakarurama. Zvinofadza kuti, kusiyana nemakrological kana mabhakiterio maitiro ekuongororwa, hutachiona hwehutachiona hahupindirani nehuwandu hwahwo.

Asi kune rumwe rutivi rwemari iyi, kana iyo ELISA kuongorora mumuenzaniso wokuratidza yakaratidza maitiro anoratidza zvakajeka kusangana kwevarwere nemapathogen, izvi hazvigoni kuva chivimbo chokuti microorganism ichiripo mumuviri womunhu. Pashure pezvose, zvirwere zvinogona kugara nguva yakareba mumuviri wemurwere kunyange pashure pokunge awana. Mhinduro isina kunaka yeE ELISA ine cheki imwechete yekuti kuvepo kwezvirwere zvekare hazvisiwo nguva dzose yakatendeseka. Nokuda kwekubuda kwemuviri wemuviri, muviri unotora nguva, ndicho chikonzero chechipiri kuongororwa kunoitwa kunyatsoongorora zvirwere zviviri kusvika kwevhiki matatu mushure mekutanga. Uye ndicho chikonzero nei ELISA kuongororwa kwehutachiona hwehutachiona hunozivikanwa husiri hwepfuura kwemavhiki matatu mushure moutachiona.

Kubatsira kwe immunoenzyme kuongororwa kwezvirwere zvinotapukira

Kuongororwa nenzira dzekudzivirira muviri kunobatsira zvakasiyana-siyana, nekuda kwaro kwave kwakakurumbira mumakore achangopfuura.

Nekubatsira kwayo, yakave yakakwanisa kuongororwa zvakasiyana-siyana zvezvirwere zvakasiyana-siyana. Somuenzaniso, zvirwere zvehutachiona (cytomegalovirus, HIV, herpes, hepatitis), zvirwere zvakawanda zvepabonde (mycoplasmosis, syphilis, chlamydia, ureaplasmosis), pamwe ne borreliosis, inouphalitis inokonzerwa nemakwenzi uye vamwe.

Nguva pfupi, iyo inokwanisika kuitisa chidzidzo, kunyanya kana ichidiwa kuti ione antigen yepathogen kana kuti kuongororwa kuti mazinga evanhu anoshanda sei kamwechete. Kazhinji, rudzi urwu rwekutsvaga runoda mazuva matatu kusvika matatu.

ELISA diagnostics inogona kushandiswa mumamiriro ezvinhu kana microscopy kana kukura zvakaoma kana zvisingaiti zvachose.

Inokwanisa kuongorora kubudirira kwemuviri wemuviri wemurwere kusvika pakuiswa kwepathogen.

Sezvo wakaziva chiyero chezvikwata zvakasiyana-siyana zveunofunoglobulin, chiremba achakwanisa kuziva paanenge ari pachiyero chirwere chiripo panguva ino, kukosha kweruzivo urwu hakugoni kuve nekufungidzirwa.

Zvose izvi zvinobatsira kuti IFA kuongororwa mubatsiri unokosha mukurapa kwemazuva ano.